Design a CRISPR construct to knockout TAT genes in cyanobacteria. Identify the optimal CRISPR plasmid backbone, design and insert the guide RNA (gRNA) into the plasmid, synthesize and insert repair ...
CRISPR-Cas9 has been widely adapted for use in transcriptional modulation and epigenetic engineering with deactivated Cas9 (dCas9) systems to enable CRISPR interference (CRISPRi) and CRISPR activation ...
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